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The -pdfsubject option sets the Subject field in a PDF file to SubjectString. This option is only available when acquiring to PDF files. By default, the Subject string in the PDF file is "None".
If SubjectString has embedded spaces, the SubjectString must be surrounded with double quotes:
twainsave -filetype pdf -pdfsubject "My Subject String"
Acquires to a PDF file and sets the PDF Subject string in the file to "My Subject String".
The Subject field is set to "None".